Medics said an Israeli air strike killed Naseem al-Kalazani, a colonel in the Hamas-run police force, when it targeted his vehicle near the al-Mawasi area in western Khan Younis, in the south of the enclave.
The attack wounded at least 17 other people, they added.
Kalazani led the anti-narcotics force in Khan Younis, Hamas sources said.
Reuters previously reported that Israel has intensified its attacks on the Hamas-run police force, which the militant group has used to reinforce its hold in the areas it controls in the strip.
Later on Wednesday, another Israeli air strike killed at least three Palestinians near al-Zeitoun neighbourhood in Gaza City, health officials at the territory's al-Ahli Hospital said, taking the day's death toll to at least four.
The Israeli military confirmed it had conducted a strike in al-Mawasi, saying it was targeting a Hamas operative.
It did not immediately respond to request for comment on the strike in al-Zeitoun.
Violence in the Gaza Strip has persisted despite an October 2025 ceasefire, with Israel conducting almost daily attacks on Palestinians.
Israel and Hamas have blamed each other for ceasefire violations.
At least 830 Palestinians have been killed since the ceasefire deal took effect, according to local medics, while Israel says militants have killed four of its soldiers over the same period.
Israel says its strikes are aimed at thwarting attempts by Hamas and other militant groups to stage attacks against its forces.
More than 72,500 Palestinians have been killed since the Gaza Strip war started in October 2023, most of them civilians, according to Gazan health authorities.
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