Kimmel, the veteran late-night comic, made several comments about the reaction to Kirk's assassination on his Monday and Tuesday night shows.
He said "many in MAGA land are working very hard to capitalise on the murder of Charlie Kirk".
The American Broadcasting Company (ABC), which has aired Kimmel's late-night show since 2003, moved swiftly after Nextstar Communications Group said it would pull the show starting on Wednesday, US time.
"'Jimmy Kimmel Live' will be pre-empted indefinitely," an ABC spokesperson said, without elaborating.
Kimmel's comments about Kirk's death "are offensive and insensitive at a critical time in our national political discourse", said Andrew Alford, president of Nexstar's broadcasting division. Nexstar operates 23 ABC affiliates.
There was no immediate comment from Kimmel.
US President Donald Trump celebrated ABC's move on his social media site Truth Social, writing: "Congratulations to ABC for finally having the courage to do what had to be done."
He also targeted two other late-night hosts, Jimmy Fallon and Seth Meyers, and said they should be cancelled too, calling them "two total losers".
Kimmel's contract is up at the end of next season, which ends in May 2026.
White House deputy chief of staff Taylor Budowich posted on social media platform X: "Welcome to Consequence Culture. Normal, commonsense Americans are no longer taking the bullshit and companies like ABC are finally willing to do the right and reasonable thing."
In his monologue on Monday, Kimmel said "we hit some new lows over the weekend with the MAGA gang desperately trying to characterise this kid who murdered Charlie Kirk as anything other than one of them and doing everything they can to score political points from it".
Kimmel said Trump's response to Kirk's death "is not how an adult grieves the murder of someone he called a friend. This is how a four-year-old mourns a goldfish, OK?"
He also said FBI chief Kash Patel has handled the investigation into the murder "like a kid who didn't read the book, BS'ing his way through an oral report".
He returned to the topic on Tuesday night, mocking Vice President JD Vance's performance as guest host for Kirk's podcast.
He said Trump was "fanning the flames" by attacking people on the left.
"Which is it, are they a bunch of sissy pickleball players because they're too scared to be hit by tennis balls, or a well-organised deadly team of commandos, because they can't be both of those things."
Kimmel, like CBS late-night host Stephen Colbert, has consistently been critical of US President Donald Trump and many of his policies on his ABC show.
CBS said earlier in 2025 it was cancelling Colbert's show at the end of this season for financial reasons, although some critics have wondered if his stance on Trump played a role.
With Reuters
