Addressing the National Press Club for the first time in her 30-year political career, the One Nation leader promised to boost regulation of AI, abolish SBS and slash taxpayer funds to the ABC.
Senator Hanson spoke about the housing crisis and immigration levels, pledging to restrict migration from "places immersed in extremism, like radical Islam".
"It's time we woke up. Western civilisation and its values are under siege," she said on Wednesday.
"While ever I lead the party, I will not walk away from my commitment to get rid of social cancer."
But the speech was interrupted by a banner being slowly unfurled onto the stage that read: "I opposed a pay rise to workers."
Press club staff quickly tore it down and allowed Senator Hanson to continue. The incident is under investigation, National Press Club chief executive Maurice Reilly told AAP.
GetUp chief executive Paul Ferris claimed responsibility for the stunt.
"Pauline Hanson has built her entire brand on being for the battlers. But her record tells a different story," he said in a statement.
"We thought the occasion deserved some honesty. So we provided it."
Senator Hanson labelled multiculturalism an "utterly flawed" policy.
"We are a multiracial society, but we must be monocultural," she said.
"Australians must live under the one cultural umbrella. Do Australians feel that the nation is losing its identity along with its values? We all know the answer to that."
Pointing to One Nation's rise in opinion polls, the firebrand senator said growing voter discontent with the status quo was behind the shift in the political landscape.
"After years of hoping for something different from the political class but getting nowhere, many Australians are looking elsewhere. I am a known quantity for these Australians," she said.
Referencing data showing an increasing number of households facing financial distress and needing help to survive, Senator Hanson said the figures were "appalling and totally un-Australian".
"What kind of Australia have we become?" she said.
The One Nation leader promised to axe SBS, saying the internet had overtaken the need for it.
Asked about the broadcaster's role in providing Australian news in about 60 different languages to help migrants integrate, Senator Hanson said she wanted newcomers to speak English on arrival.
The ABC would still exist, but the media outlet would be greatly scaled back, with taxpayer money only funding regional operations.
"In the cities, which are already saturated with media outlets across the political spectrum, the ABC will only be a subscription service," she said.
Senator Hanson said a One Nation government would introduce nuclear energy into the national grid and while environmental standards had to be met, Australia could not do without coal and gas.
She said her party would end "renewable energy bribery" by scrapping grants, tax incentives, concessions, and any taxpayer money that "sponsors the whole net zero hoax".
In a bid to rein in spending, Senator Hanson promised to dismantle a number of government departments including those relating to climate change and Indigenous issues.
She would also get rid of federal health and education departments to remove what she described as duplication with the states.
Concerns over artificial intelligence also featured in the speech as the politician warned governments should not leave the industry to self-regulate.
"If AI is to be successfully deployed, public trust is a prerequisite," she said.
The political leader also attacked the "transgender insurgency" saying the "movement is a militant force right throughout society and must be confronted".
Dozens of demonstrators shouting "Pauline Hanson go to hell" gathered outside the Canberra venue for the speech.
